REGENERATIVE MEDICINE
HIFU is a revolutionary non-invasive treatment that uses ultrasound energy to lift and tighten the skin. This method is particularly effective for addressing sagging skin and loss of elasticity in the face and neck. By penetrating deep into the tissue, HIFU promotes collagen production, leading to firmer, more youthful skin over time. Patients appreciate the long-lasting results and the absence of surgery, making HIFU a popular option for those seeking a facelift alternative.
Introduction
At Dr. Nancy London Aesthetic Clinic, we bring the latest advancements in regenerative medicine to help you achieve naturally youthful, rejuvenated skin and enhanced overall wellness. By leveraging the body’s ability to heal and regenerate, our cutting-edge treatments stimulate collagen production, repair damaged tissues, and restore vitality to the skin.
Our regenerative medicine program combines science-backed treatments such as autologous biologics (PRP, PRF, exosomes, and stem cells), growth factors and peptides, skin boosters, and energy-based devices with regenerative properties. These treatments go beyond traditional aesthetic procedures, working at the cellular level to promote long-lasting skin renewal, tissue repair, and anti-aging benefits. Whether you’re looking to improve skin quality, boost hydration, accelerate wound healing, or restore hair density, our expert team offers customized solutions to meet your unique needs.
“ Very warm welcome. I was very pleased with the outcome of my treatment. Looking forward to Saturday and next Monday when I will ve having more treatment. ”
Gillian
Science Behind Regenerative Medicine Treatments
Autologous biologics involve using the body’s natural healing components to regenerate skin and promote tissue repair. Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) are derived from the patient’s own blood and contain growth factors that stimulate collagen production and cellular renewal. Exosomes and stem cells take regenerative therapy further by delivering powerful bioactive molecules that accelerate healing, improve skin elasticity, and restore youthful volume.
Regenerative Medicine Treatments
- Autologous Biologics (PRP, PPP, PRF, Stem Cells)
- Platelet-Rich Plasma (PRP)
- What it is: A concentration of platelets from your blood, rich in growth factors.
- How it works: Injected or applied topically (via microneedling) to stimulate collagen and elastin.
- Key Uses: Facial rejuvenation, hair restoration, scar revision, under-eye dark circles.
- Platelet-Poor Plasma (PPP)
- What it is: The plasma layer left after PRP extraction, containing fewer platelets but still beneficial proteins.
- How it works: Used as a hydrating skin booster or mixed with PRP for enhanced effects.
- Key Uses: Skin hydration, fine line reduction, post-laser recovery.
- Platelet-Rich Fibrin (PRF)
- What it is: An advanced version of PRP with a fibrin matrix for slower growth factor release.
- How it works: Provides longer-lasting regenerative effects (up to 12 months).
- Key Uses: Facial volumizing, acne scars, wound healing, hair growth.
- Stem Cell Therapy (from Fat or Bone Marrow)
- What it is: Undifferentiated cells that can regenerate damaged tissues.
- How it works: Extracted from fat (adipose-derived stem cells) or bone marrow, then injected into target areas.
- Key Uses: Deep skin rejuvenation, joint repair, breast/natural buttock augmentation.
Growth Factors & Peptides (EGF, FGF, TGF-B, GHK-Cu, Matrikines)
Key Players in Cellular Regeneration
- Growth Factors
- EGF (Epidermal Growth Factor)
- Function:Stimulates keratinocyte proliferation
- Aesthetic Benefits:Accelerates wound healing, reduces fine lines
- Clinical Uses:Post-procedure recovery, anti-aging protocols
- FGF (Fibroblast Growth Factor)
- Function:Promotes angiogenesis and collagen synthesis
- Aesthetic Benefits:Improves skin thickness and elasticity
- Clinical Uses:Atrophic scarring, deep wrinkles
- TGF-β (Transforming Growth Factor Beta)
- Function:Regulates extracellular matrix production
- Aesthetic Benefits:Reduces fibrosis, improves skin texture
- Clinical Uses:Hypertrophic scars, skin remodeling
- Bioactive Peptides
- Copper Peptides
- Matrixyl®
- Argireline®
Mechanism:Signal collagen production, inhibit muscle contraction
Synergy with Autologous Biologics
Combination | Enhanced Benefit |
PRP + EGF | Supercharged epithelial regeneration |
PRF + FGF | Sustained collagen stimulation |
Stem Cells + TGF-β | Optimal tissue remodeling |
Treatment Protocol
Administration Methods
- Microinjection (mesotherapy)
- Topical + Needle Delivery (after microneedling)
- Biologic Cocktails (custom blended)
Treatment Plan
Concern | Recommended Protocol | Sessions | Frequency |
Facial Rejuvenation | GF Cocktail + PRF | 3-5 | Every 4 weeks |
Acne Scarring | FGF + Microneedling | 4-6 | Every 6 weeks |
Hair Restoration | EGF + PRP | 6-8 | Monthly |
Neck/Decolletage | TGF-β + HA | 3 | Every 5 weeks |
The Science Behind Results
- Molecular Pathways Activated:
- MAPK/ERK (cell proliferation)
- SMAD (collagen production)
- PI3K/AKT (cell survival)
- Histologic Changes:
- 40% ↑ in collagen density (12 weeks)
- 30% ↑ in epidermal thickness
Safety Profile
- Side Effects: Mild erythema (12-24 hrs)
- Contraindications: Active skin infections
- Anesthesia: Topical numbing only
Celebrity Adoption
- Nicole Kidman: EGF treatments for red carpet prep
- Shah Rukh Khan: PRP+GF combo for anti-aging
- Gwyneth Paltrow: TGF-β for scar revision
Skin Boosters (HA, PN, PDRN, Amino Acids, Vitamins)
Skin boosters are injectable bio-stimulators that deliver high-performance nutrients directly into the skin to:
✔ Restore hydration (HA attracts 1000x its weight in water)
✔ Stimulate collagen & elastin (PN & PDRN repair DNA damage)
✔ Brighten & even skin tone (vitamins & amino acids nourish cells)
✔ Enhance skin elasticity (works synergistically with PRP/PRF)
Key Ingredients & Their Benefits
Ingredient | Key Benefit | Best For |
Hyaluronic Acid (HA) | Deep hydration, plumping fine lines | Dry, dull skin |
Polynucleotides (PN) | DNA repair, wound healing, collagen boost | Aging, sun damage |
PDRN (Salmon DNA) | Anti-inflammatory, tissue regeneration | Acne scars, redness |
Amino Acids | Strengthens skin barrier, detoxifies | Sensitive, reactive skin |
Vitamins (B, C, E) | Antioxidant protection, brightening | Pigmentation, dullness |
How Skin Boosters Work
- Micro-injections deliver a customized cocktail into the mid-dermis.
- HA & PN create an instant hydration surge while stimulating fibroblasts.
- PDRN & growth factors (from PRP/PRF) accelerate repair over 4-6 weeks.
- Results improve progressively—peak at 4-8 weeks post-treatment.
Combination Therapies for Enhanced Results
- PRP + HA Skin Boosters = “Liquid Facelift” effect
- PRF + PN = Long-lasting collagen remodeling
- Stem Cells + PDRN = Extreme regeneration for scars/stretch marks
Treatment Areas
✅ Face (cheeks, under-eyes, nasolabial folds)
✅ Neck & décolletage
✅ Hands
✅ Scalp (for thinning hair)
Treatment Protocol
Concern | Sessions Needed | Gap Between Sessions | Downtime |
Basic Hydration | 1-2 | 4 weeks | 24 hrs redness |
Anti-Aging | 3-4 | 4-6 weeks | 2-3 days swelling |
Acne Scars | 3-5 | 6 weeks | 3-5 days micro-crusts |
Hair & Scalp | 4-6 | 4 weeks | None |
Science Behind Skin Boosters
- HA: Binds water + triggers collagen via CD44 receptor activation.
- PN/PDRN: Upregulates TGF-β for fibroblast proliferation.
- PRP/PRF: Adds VEGF/EGF for angiogenesis & tissue repair.
Side Effects & Safety
- Mild swelling/redness (12-48 hrs).
- Rare bruising (avoid blood thinners pre-treatment).
- No anesthesia needed (topical numbing cream used).
Pre/Post-Care Instructions
Before:
- Avoid alcohol, NSAIDs, and sun exposure 48 hrs prior.
After: - No makeup/sweating for 24 hrs.
- Use SPF 50+ and hyaluronic serum.
Celebrities Who Love Skin Boosters
- Jennifer Lopez – Uses HA + PRP for “glass skin” glow.
- Sonam Kapoor – Swears by PN boosters for red-carpet radiance.
- Tom Cruise – Reported to use PDRN for scarless healing.
Energy-Based Devices with Regenerative Properties
- RF Microneedling: Uses tiny needles with radiofrequency to stimulate collagen production and improve skin texture.
- Fractional CO2 or Erbium YAG Laser: Resurfaces the skin by creating controlled micro-injuries that trigger collagen remodeling.
- HIFU (High-Intensity Focused Ultrasound): Uses ultrasound energy to lift and tighten skin at a deeper level.
Key Uses & Benefits:
- Skin tightening and firming
- Improves scars, fine lines, and wrinkles
- Stimulates natural collagen and elastin production
Target Areas: Face, neck, body
Sessions Required: 1-3 sessions
Gap Between Sessions: 4-6 weeks
Downtime: Varies by treatment, typically 2-7 days
Side Effects: Redness, peeling, mild swelling
Anesthesia: Numbing cream or local anesthesia (if required)
Pre/Post Treatment Care: Avoid sun exposure, use gentle skincare
- Exosomes are extracellular vesicles that act as messengers, delivering proteins and RNA to enhance cellular regeneration and reduce inflammation.
Exosomes are nanoscopic vesicles (30-150 nm) released by stem cells that carry proteins, growth factors, and genetic material to stimulate tissue repair, collagen production, and cellular regeneration. Unlike traditional stem cell therapy, exosomes provide all the regenerative benefits without needing live cells, making them safer and more versatile.
How Exosome Therapy Works
- Signalling & Repair– Exosomes deliver instructions to aging or damaged cells, triggering natural healing.
- Collagen & Elastin Boost– Stimulates fibroblasts to produce youthful skin structure.
- Anti-Inflammatory Effects– Reduces redness, acne scars, and pigmentation.
- Angiogenesis– Improves blood flow for a radiant glow.
Key Uses & Benefits
✅ Deep Wrinkle Reduction – Smoothes fine lines and creases.
✅ Acne Scar & Pigmentation Treatment – Repairs damaged skin texture.
✅ Hair Regrowth – Revitalizes dormant hair follicles.
✅ Post-Laser & Microneedling Enhancement – Speeds up recovery and improves results.
✅ Non-Surgical Facial Rejuvenation – Natural alternative to fillers and facelifts.
Exosomes vs. PRP, PRF, PPP & Stem Cells
Treatment | Source | Key Benefit | Best For |
Exosomes | Lab-cultured stem cell secretions | Ultra-potent regenerative signals | Advanced aging, scars, hair loss |
PRP | Patient’s blood (platelets) | Growth factor stimulation | Mild-moderate skin rejuvenation |
PRF | Patient’s blood (fibrin matrix) | Slow-release growth factors | Long-lasting volumizing |
PPP | Patient’s blood (plasma) | Hydration & skin healing | Fine lines, dryness |
Stem Cells | Patient’s fat/bone marrow | Live cell regeneration | Deep tissue repair, volume loss |
Treatment Process
- Consultation– Assess skin/hair concerns.
- Application– Exosomes are injected, applied topically after microneedling, or combined with PRP/PRF.
- Post-Treatment– Minimal downtime (24-48 hrs redness).
Treatment Plan & Sessions
Concern | Sessions Needed | Gap Between Sessions | Results Timeline |
Facial Rejuvenation | 2-3 | 4-6 weeks | 1-3 months |
Hair Restoration | 3-6 | 4-8 weeks | 6-12 months |
Acne Scars | 3-4 | 6-8 weeks | 3-6 months |
Neck/Hand Rejuvenation | 2-3 | 6 weeks | 2-4 months |
Side Effects & Safety
- Minimal downtime(slight redness/swelling for 1-2 days).
- No anesthesia required(topical numbing cream used).
- No risk of rejection(exosomes are non-cellular).
Pre & Post-Treatment Care
Before:
- Avoid blood thinners, alcohol, and sun exposure.
After: - Use gentle skincare, avoid sun, and stay hydrated.
- No intense exercise for 24 hours.
Celebrities Who Use Exosome & Regenerative Therapies
- Gwyneth Paltrow– Advocates for cutting-edge regenerative treatments.
- Bryan Johnson– Tech entrepreneur using exosomes in his anti-aging routine.
- Deepika Padukone– Rumored to use PRP/exosome facials for red-carpet glow.
Regenerative Biologics for Structural Rejuvenation
What Are Biostimulators?
Biocompatible injectables that trigger your skin to produce its own collagen for gradual, natural-looking volume restoration:
Biostimulator | Mechanism | Duration | Best For |
PLLA (Sculptra®) | Poly-L-lactic acid microspheres stimulate fibroblasts | 2-3+ years | Full face volumization |
CaHA (Radiesse®) | Calcium hydroxylapatite creates collagen scaffolding | 12-18 months | Midface, hands, jawline |
Ellansé® | Polycaprolactone (PCL) provides immediate+long-term correction | 1-4 years (depending on formulation) | Deep folds, facial contours |
Synergy with Regenerative Biologics
Enhanced Protocols
- PLLA + PRF
- Benefit:PRF growth factors accelerate collagen response
- Ideal For:HIV-related lipoatrophy, age-related volume loss
- CaHA + PRP
- Benefit:PRP improves skin quality while CaHA restores structure
- Ideal For:Marionette lines, hand rejuvenation
- Ellansé® + Stem Cells
- Benefit:Stem cells enhance tissue regeneration around PCL matrix
- Ideal For:Post-weight loss facial remodeling
Treatment Process
Step-by-Step Protocol
- Consultation
- 3D facial analysis to identify volume deficit patterns
- Preparation
- Blood draw for PRP/PRF (if combining)
- Topical anesthesia application
- Injection Technique
- PLLA:Deep subcutaneous fanning technique
- CaHA:Supraperiosteal placement for structural support
- Ellansé®:Precise depot technique
- Post-Treatment
- 5-minute massage (critical for PLLA)
- LED therapy to reduce inflammation
Treatment Plans & Results Timeline
Product | Sessions Needed | Interval | First Results | Peak Results |
PLLA | 3 | 4-6 weeks | 6 weeks | 3-6 months |
CaHA | 1-2 | 6 months | Immediate | 4-8 weeks |
Ellansé® S | 1 | N/A | Immediate | 1 month |
Ellansé® M/E | 1-2 | 12 months | Immediate | 3 months |
Science Behind Biostimulation
Cellular Mechanisms
- PLLA:Degrades into lactic acid → stimulates Type I collagen via TGF-β pathway
- CaHA:Acts as “microscaffold” for neocollagenesis (up to 65% increase at 6 months)
- Ellansé®:PCL matrix induces controlled foreign body response → collagen deposition
Histologic Evidence
- 82%increase in collagen density with PLLA at 12 months (Histologic study)
- 5mmdermal thickening with CaHA at 6 months (Ultrasound data)
Safety & Downtime
Side Effect Profile
Concern | PLLA | CaHA | Ellansé® |
Swelling | 3-7 days | 2-5 days | 3-7 days |
Nodules | <5% risk | <3% risk | <1% risk |
Bruising | Mild | Moderate | Mild |
Pre/Post-Care
Before:
- Avoid NSAIDs 1 week prior
- Start arnica supplements 3 days prior
After:
- PLLA:5/5/5 massage rule (5x/day for 5 days for 5 mins)
- CaHA:Avoid pressure on treated areas x48hrs
- Ellansé®:No strenuous exercise x72hrs
Celebrity Adoption
Hollywood
- Madonna:PLLA for “invisible volumization”
- Kristen Bell:CaHA for natural jawline definition
Bollywood
- Kareena Kapoor:Ellansé® for post-pregnancy facial restoration
Ranveer Singh: PRP+PLLA combo for facial sculpting
- REGENERATIVE TOPICAL THERAPIES: MEDICAL-GRADE SERUMS & CREAMS
Science-Backed Active Ingredients
- Retinoids (Tretinoin, Retinaldehyde)
- Mechanism:Binds RAR/RXR receptors to increase cellular turnover
- Clinical Benefits:
- Gold standard for anti-aging (↑ collagen I/III)
- Treats acne by normalizing keratinization
- Enhanced Protocol:PRP + tretinoin increases collagen 37% more than tretinoin alone
- Vitamin C (L-Ascorbic Acid, THD Ascorbate)
- Optimal Formulation:20% L-AA + vitamin E + ferulic acid (pH 3.2)
- Proven Effects:
- 62% reduction in UV-induced erythema
- 40% brighter skin in 12 weeks
- Niacinamide (5-10%)
- Multitasking Benefits:
- ↓ Transepidermal water loss by 24%
- ↓ Melanosome transfer (pigmentation)
- ↑ Ceramide synthesis
- Coenzyme Q10 (Nanoemulsified)
- Mitochondrial Protection:
- Neutralizes free radicals in dermal fibroblasts
- Preserves collagen from glycation
- Ceramide Complex (3:1:1 Ratio)
- Barrier Repair:
- Restores lipid matrix architecture
- Essential for post-procedure recovery
- Peptides (Palmitoyl Pentapeptide-4, Acetyl Hexapeptide-8)
- Targeted Signaling:
- Stimulates collagen without irritation
- Relaxes expression lines (topical “Botox” effect)
Synergy with Regenerative Biologics
Combination | Enhanced Benefit | Ideal For |
PRP + Retinoids | Accelerates retinization process | Photoaging |
PRF + Vitamin C | Prolongs antioxidant protection | Hyperpigmentation |
Stem Cell CM + Peptides | 3X collagen stimulation | Neck/décolletage |
Treatment Protocols
Clinical Application Methods
- Post-Microneedling Delivery(0.5mm depth)
- Iontophoresis(for charged molecules like vitamin C)
- Occlusive Mask Technique(for maximum penetration)
At-Home Regimen Timeline
Phase | Duration | Key Actives |
Barrier Repair | Weeks 1-2 | Ceramides, Niacinamide |
Retinization | Weeks 3-8 | Low-dose tretinoin (0.025%) |
Maintenance | Week 9+ | Peptides, Antioxidants |
Scientific Validation
Key Clinical Studies
- Retinoids:05% tretinoin ↑ epidermal thickness by 25% (12 months)
- Vitamin C:Daily use ↓ fine lines by 27% (UV photography)
- Niacinamide:4% reduction in sebum production (sebometry)
Histologic Improvements
- 40%↑ in dermal collagen (retinoid + PRP combo)
- 3xfaster wound healing (ceramide-based protocols)
Safety & Tolerability
Side Effect Management
Ingredient | Potential Reaction | Solution |
Retinoids | Erythema, peeling | “Sandwich method” (moisturizer first) |
Vitamin C | Tingling (pH-related) | Start with 10% concentration |
Niacinamide | Flushing (rare) | Take with aspirin pre-application |
Pre/Post-Procedure Care
Before Treatment:
- Discontinue retinoids 3 days prior to procedures
- “Prep” skin with ceramide moisturizer
After Treatment:
- Day 1-3:Healing ointment + growth factors
- Day 4-7:Restart actives at 50% frequency
Celebrity Adoption
Hollywood
- Angelina Jolie:Uses medical-grade retinaldehyde for red carpet prep
- Jennifer Aniston:Vitamin C + PRP “glow boost” regimen
Bollywood
- Deepika Padukone:Niacinamide + stem cell conditioned media for pigmentation
- Alia Bhatt:Ceramide-dominant barrier repair protocol
Mesotherapy Cocktails
What Is Mesotherapy?
Mesotherapy involves microinjections of customized nutrient-rich solutions into the mesoderm (middle layer of skin) to:
✔ Stimulate collagen & elastin
✔ Restore hydration & radiance
✔ Reverse signs of aging
✔ Enhance skin texture & tone
Premium Mesotherapy Cocktails We Use
- NCTF® (New Cellular Treatment Factor) by Filorga
- What It Contains:59 active ingredients (vitamins, amino acids, minerals, coenzymes, nucleic acids, antioxidants)
- How It Works:Replenishes cellular energy (ATP) and boosts fibroblast activity
- Best For:Global anti-aging, dull skin, fine lines
- Jalupro® (Hyaluronic Acid + Amino Acid Complex)
- Key Ingredients:Hyaluronic acid, glycine, proline, lysine, leucine
- Mechanism:Directly supplies building blocks for collagen synthesis
- Best For:Acne scars, deep wrinkles, skin laxity
- Revitacare® (Growth Factors + Peptides)
- Active Components:Epidermal growth factor (EGF), copper peptides
- Action:Stimulates tissue repair and angiogenesis
- Best For:Post-procedure healing, hyperpigmentation
- Cytocare® (Cytokines + Hyaluronic Acid)
- Unique Feature:502 & 532 formulations for different skin depths
- Benefits:Improves skin density and elasticity
- Best For:Neck, décolletage, under-eye rejuvenation
Synergy with Autologous Biologics
Combination | Enhanced Benefit | Best For |
NCTF + PRP | 2X collagen stimulation | Full-face rejuvenation |
Jalupro + PRF | Longer-lasting amino acid release | Acne scarring |
Revitacare + PPP | Hydration + epidermal repair | Sensitive skin |
Cytocare + Stem Cells | Deep regenerative effects | Neck & hands |
Treatment Protocol & Expected Results
Target Areas
✅ Face (cheeks, forehead, nasolabial folds)
✅ Under-eye area (dark circles, fine lines)
✅ Neck & décolletage
✅ Scalp (for hair thinning)
Treatment Plan
Concern | Recommended Cocktail | Sessions Needed | Frequency | Results Timeline |
Anti-Aging | NCTF + PRP | 4-6 | Every 2-4 weeks | 3-6 months |
Acne Scars | Jalupro + PRF | 5-8 | Every 4 weeks | 6-12 months |
Hydration Boost | Cytocare 502 + PPP | 3 | Every 3 weeks | Immediate + long-term |
Hair Growth | NCTF + PRP Scalp | 6-8 | Monthly | 4-6 months |
Science Behind Mesotherapy
- Fibroblast Activation:NCTF increases Type I collagen by 68% (histological study)
- Cellular Energy Boost:Amino acids in Jalupro enhance ATP production
- Growth Factor Signaling:Revitacare upregulates TGF-β for tissue repair
- Hyaluronic Acid Hydration:Cytocare binds 1000x its weight in water
Safety, Downtime & Aftercare
Side Effects (Minimal & Temporary)
✔ Mild redness (12-24 hrs)
✔ Small bumps (resolve in 1-2 days)
✔ Rare bruising (avoid blood thinners pre-treatment)
Anesthesia
- Topical numbing cream (20-30 mins before treatment)
Pre & Post-Treatment Care
Before:
- Avoid alcohol & NSAIDs 48 hrs prior
- No retinoids 3 days before
After:
- No makeup/sweating for 24 hrs
- Use SPF 50+ and hyaluronic acid serum
- Gentle cleansing for 48 hrs
Celebrity Endorsements
- Kim Kardashian– Loves NCTF “liquid facelifts” for red-carpet glow
- Sonam Kapoor– Uses Jalupro for acne scar treatment
- Brad Pitt– Reported to use Cytocare for neck rejuvenation
Frequently Asked Questions
A combination of PRP/PRF with RF Microneedling or skin boosters provides excellent results for anti-aging.
Most treatments are well tolerated. A numbing cream can be applied for added comfort.
Results develop gradually over a few weeks, with optimal improvement seen after multiple sessions.
Yes! Combining PRP, exosomes, and energy-based treatments often enhances results.
Book your consultation today!
